Journal: Biosensors & bioelectronics

Article Title: Analysis of Inflammatory Biomarkers by Arrayed Imaging Reflectometry

doi: 10.1016/j.bios.2011.02.025

Figure Lengend Snippet: A representative concentration profile showing the response of an AIR microarray to varying concentrations of Tissue Factor (TF) in buffer containing a background of 10 mg/mL BSA. The lower limit of detection (LOD) for this analyte was determined to be below 1 pg/mL, as our unexposed control chip has zero normalized reflectance on this scale.

Article Snippet: All images were acquired in TIFF format on a commercially available AIR Microarray Reader (Adarza BioSystems) operating with 632.8 nm illumination at an incident angle of 70.75 degrees.

Techniques: Concentration Assay, Microarray, Control

Journal: Journal of Immunological Methods

Article Title: Agrodiag PorCoV: A multiplex immunoassay for the differential diagnosis of porcine enteric coronaviruses

doi: 10.1016/j.jim.2020.112808

Figure Lengend Snippet: Overview of the design, fabrication and use of the AgroDiag porcine enteric coronavirus (PEC) multiplex planar immunoassay. (A) Manufacturing steps include functionalization of the wells of a microplate with a dextran layer before printing of the protein array in triplicate. (B) The assay protocol and working principle is similar to the solid-phase standard indirect ELISA. After the three typical incubation steps (serum sample, conjugate, and substrate), followed by microplate reading. The reaction was visualized as brown spots which intensity correlated with antibody levels to specific viral antigen target in the array. Four results are obtainable depending on incubation with a negative control, or samples from PDCoV, PEDV, TGEV inoculation groups. Scale bar corresponds to 1 mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Supplementary Fig. S1 Supplementary Fig. S2 AgroDiag PEC multiplex immunoassay raw data obtained with the microplate reader (CLAIR Sensovation®) on serum samples, tested in duplicate, collected at day post-inoculation −7, 0, 7, 14, 21, 28, 35, and 42 from pigs ( n = 12, per group) experimentally inoculated with: (A) culture media (NEG); (B) porcine deltacoronavirus (PDCoV); (C) porcine epidemic diarrhea virus (PEDV); (D) transmissible gastroenteritis virus (TGEV) Purdue; (E) TGEV Miller.

Techniques: Multiplex Assay, Protein Array, Indirect ELISA, Incubation, Negative Control